Cellular Senescence
Cellular senescence is physiologically characterized by functional decline and hypometabolism, such as cell cycle arrest, loss of cell replication ability, decreased responsiveness to mitogenic stimuli, and altered responsiveness to pro-apoptotic factors. Intracellular enzyme active centers are oxidized, enzyme activity is reduced, and protein synthesis is decreased. Senescent cells are no longer able to replicate, but they remain metabolically active and are positive for senescence-associated β-galactosidase activity, which is considered a biomarker of cellular senescence.
Features and Advantages
- Simple and optimized procedure.
- Fast and convenient.
- Suitable for aging detection of cultured cells and tissue sections.
- Cell senescence β-galactosidase staining kit only stains senescent cells, and does not stain pre-senescence cells (senescent cells), quiescent cells (static cells), immortal cells (immortal cells) or tumor cells.
Senescence β-Galactosidase Staining Kit provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated β-galactosidase (SA-β-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-β-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells.
Kit components
• 10×Fixation Buffer
• 10×PBS
• Reagent A
• Reagent B
• Reagent C
• X-Gal solution
• 10×Fixation Buffer
• 10×PBS
• Reagent A
• Reagent B
• Reagent C
• X-Gal solution