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[DL4000] ExcelDye™ 6X DNA Loading Dye, Tri-color, 5 ml x 2
Description The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease. DATASHEET |
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TBE Buffer, 10X, Molecular Biology Grade, 1L
A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3. Tris, borate, and ethylenediaminetetraacetic acid (TBE) buffer is used to perform agarose gel electrophoresis of DNA and RNA. It consists of a weak acid that exists in neutral and anionic forms (e.g., COOH and COO- species). Tris is a weak base, which occurs in either neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions maintain a low conductivity medium, transmit the electrical current, and buffer the pH. Ethylenediaminetetraacetic (EDTA) although not essential is added as a preventative since it chelates Mg2+ ions and inactivates potential DNA nucleases. TBE allows an effective resolution of DNA fragments and slightly improves the separation of smaller fragments.[1] It also stabilizes nucleic acids against enzymatic degradation.[2] PRODCUT DETAIL |
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TAE Buffer, 10X, Molecular Biology Grade, 1L
General descriptionTris, acetate, and ethylenediaminetetraacetic acid (TAE) buffer consists of tris(2-amino-2-[hydroxymethyl]-1,3-propanediol) base, acetate, and ethylenediaminetetraacetic acid (EDTA). This is one of the most used running buffers in nucleic acid electrophoresis. TAE buffer improves the separation or resolution of large DNA fragments.[1]TAE, a standard buffer, is mainly used to perform agarose gel electrophoresis of DNA and RNA in slab gels. TAE is one of the commonly used buffers in every biochemistry and molecular biology laboratory.[2] PRODCUT DETAIL |
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Tris-EDTA buffer solution, 100ML (93283-100ML)
Tris-EDTA buffer solution has been used as a component in Tris-EDTA (TE) buffer to resuspend DNA pellets for total DNA extraction from herbage samples and single-stranded carrier DNA extraction from salmon sperm. Ethylenediaminetetraacetic acid (EDTA) and its salts are used in a variety of medical and pharmaceutical applications. It is a calcium chelator that also acts as an anticoagulant and a detoxicant in the case of heavy metal poisoning. EDTA is frequently used in analytical chemistry for complexometric titrations and several other applications. It is used as a food additive to bind metal ions due to its low toxicity. Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer PRODUCT DETAIL |
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Nuclease-Free Water, for Molecular Biology (W4502)
ApplicationWater has been used
PRODUCT DETAIL |
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Lysozyme, LDB0308-1G, L8120-1G
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. |
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Proteinase K, FAPNK001-11
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. RNase A BF-RNAS-11mg
RNase A solution has been used: RNase A has been used: in apoptosis assay of Hep-2 cells, for the colorimetric quantification of methylated DNA, for DNA and RNA extraction, in DNA laddering analysis, in cell cycle analysis, as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry Zymolase / Lyticase (L8141)
Lyticase from Arthrobacter luteus has been used to lyse the fungal cell wall for DNA isolation. |
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2-Mercaptoethanol/β-Mercaptoethanol, M3148-25ML
for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) |
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2-Propanol/Isopropanol (1.09634.1000)
for analysis EMSURE® ACS,ISO,Reag. Ph Eur |
RNaseZAP™ (R2020-250ML)
RNaseZAP™ is a purifying agent used for eliminating RNase contamination from glassware, plastic surfaces, reaction vessels, countertops and pipettors. It is also used for effective removal of RNase residues from microcentrifuge tubes without inhibiting subsequent enzymatic reactions
RNaseZAP™ is a purifying agent used for eliminating RNase contamination from glassware, plastic surfaces, reaction vessels, countertops and pipettors. It is also used for effective removal of RNase residues from microcentrifuge tubes without inhibiting subsequent enzymatic reactions
LookOut® DNA Erase (L8917-250ML)
LookOut™ DNA Erase is a potent, ready-to-use solution for rapid DNA decontamination of surfaces in laboratories. This reagent is characterized by its high efficiency. The decontamination spray completely destroys DNA within 60 seconds of surface treatment. The solution contains a unique combination of DNA destroying and surface active agents.
LookOut™ DNA Erase is a potent, ready-to-use solution for rapid DNA decontamination of surfaces in laboratories. This reagent is characterized by its high efficiency. The decontamination spray completely destroys DNA within 60 seconds of surface treatment. The solution contains a unique combination of DNA destroying and surface active agents.