Universal WB Toolkit (20T)
​KTD106-EN

Western blotting is a method for detecting a certain protein in complex samples based on the specific binding of antigen and antibody. Due to the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassays, western blotting has become a routine technique for protein analysis.
Abbkine has developed a Universal WB Toolkit. After careful design and research, one-stop operation can meet the needs of routine WB experiments and obtain excellent experimental results.
Kit components
• Lysis Buffer-2 mL
• Running Buffer (10×)-100 mL
• Transfer Buffer (10×)-100 mL
• TBST (10×)-100 mL
• BSA-3 g
• PVDF Membrane (8.5 cm×6 cm)-2
• SuperKine™ West Femto Maximum Sensitivity Substrate A-4 mL
• SuperKine™ West Femto Maximum Sensitivity Substrate B-4 mL
• WB Positive Control-40 μL
• Anti-GAPDH Mouse Monoclonal Antibody (2B5)-6 μL
• Colorcode Prestained Protein Marker (10-180 kDa)-5 μL
• HRP, Goat Anti-Mouse IgG-3 μL
• HRP, Goat Anti-Rabbit IgG-3 μL
• SDS-PAGE Loading Buffer (5×)-1 mL
Features & Benefits
• Standardization of results: A ready-to-use WB positive control, which can be directly loaded to provide a real WB experimental reference;
• Ultra-high sensitivity: SuperKine™ West Femto Maximum Sensitivity Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments;
• Convenient and ready to use: Easy to operate, ready-to-use reagents, no need to prepare complex reagents, you can start the experiment right out of the box;
• High efficiency Lysate: Optimized lysate for sample WB needs.
Publication
Comparative efficacy evaluation of disinfectants against severe acute respiratory syndrome coronavirus-2(KTD106-EN-6.9)Author：Lee, Gun-Hee, et al. Publication name：Journal of Hospital Infection IF：6.9

 Constructing a prognostic risk model for Alzheimer's disease based on ferroptosis(KTD106-EN-4.8)Author：Wang, Xiao-Li, et al. Publication name：Frontiers in Aging Neuroscience IF：4.8