Based on the tedious and time-consuming operation of traditional IF experiments, a wide variety of reagents and supplies, and poor stability and reliability of experimental results, Abbkine has developed a Universal IF Toolkit.

Applications: Immunofluorescence detection of tissue and cell samples

Universal IF Toolkit (Anti-Rabbit Dylight 488)

Kit components
• Immunostaining Permeabilization Buffer-100 mL
• EDTA Antigen Retrieval Solution pH 8.0 (20×)-100 mL
• PBS (20×)-100 mL
• Goat Serum Blocking Buffer-20 mL
• Antibody Wash Buffer (20×)-25 mL
• Antibody Dilution Buffer-50 mL
• DyLight 488, Goat Anti-Rabbit IgG-135 μL
• DAPI (500×)-100 μL
• SuperKine™ Enhanced Antifade Mounting Medium-10 mL
Features & Benefits
• ①The operation is convenient, the components are complete, and there is no need to worry about complex reagents, and the experiment can be started immediately after opening the box;
• ②Provide optimized antibody diluent and SuperKine™ Enhanced Antifade Mounting Medium, which has strong anti-quenching effect and maintains excellent fluorescence intensity;
• ③Provide Dylight series secondary antibodies with stronger fluorescence intensity and higher specificity;
• ④DyLight 488, Goat Anti-Rabbit IgG can be used to detect the corresponding primary antibody of rabbit origin, and the observed color is bright green.

Universal IF Toolkit (Anti-Mouse Dylight 488)

Kit components
• Immunostaining Permeabilization Buffer-100 mL
• EDTA Antigen Retrieval Solution pH 8.0 (20×)-100 mL
• PBS (20×)-100 mL
• Goat Serum Blocking Buffer-20 mL
• Antibody Wash Buffer (20×)-25 mL
• Antibody Dilution Buffer-50 mL
• DyLight 488, Goat Anti-Mouse IgG-100 μL
• DAPI (500×)-100 μL
• SuperKine™ Enhanced Antifade Mounting Medium-10 mL
Features & Benefits
• ①The operation is convenient, the components are complete, and there is no need to worry about complex reagents, and the experiment can be started immediately after opening the box;
• ②Provide optimized antibody diluent and SuperKine™ Enhanced Antifade Mounting Medium, which has strong anti-quenching effect and maintains excellent fluorescence intensity;
• ③Provide Dylight series secondary antibodies with stronger fluorescence intensity and higher specificity;
• ④DyLight 488, Goat Anti-Mouse IgG can be used to detect the corresponding primary antibody of rabbit origin, and the observed color is bright green.
Publication:
​TGF-β1-induced bone marrow mesenchymal stem cells (BMSCs) migration via histone demethylase KDM6B mediated inhibition of methylation marker H3K27me(KTD108-EN-7.127)Author：  He Q,  Shi J,  Liu W Publication name：Cell Death Discovery IF：7.127